Make up following solutions:
(maybe stored for months at room temp.) Ratios are v/v.
Fixative: |
40% methanol; 10% acetic acid; 50% water |
Wash Sol.: |
30% ethanol in water |
Water: |
d.i. water |
Reductant: |
200 mg sodium thiosulfate in 1 liter water |
Silver stain: |
2 g silver nitrate; 200 uL formaldehyde in 1 liter of water. Wrap bottle in aluminum foil to prevent light from getting in. |
Developer: |
30 g sodium carbonate; 5 mg sodium thiosulfate; 500 uL formaldehyde in 1 L water |
Stop Sol.: |
5% acetic acid in water |
Begin staining (Procedure is for mini-gel; takes about 2 hours; use longer incubation times for thicker gels)